Variability of an Early Developmental Cell Population Underlies Stochastic Laterality Defects.
Cell Rep
; 34(2): 108606, 2021 01 12.
Article
em En
| MEDLINE
| ID: mdl-33440143
ABSTRACT
Embryonic development seemingly proceeds with almost perfect precision. However, it is largely unknown how much underlying microscopic variability is compatible with normal development. Here, we quantify embryo-to-embryo variability in vertebrate development by studying cell number variation in the zebrafish endoderm. We notice that the size of a sub-population of the endoderm, the dorsal forerunner cells (DFCs, which later form the left-right organizer), exhibits significantly more embryo-to-embryo variation than the rest of the endoderm. We find that, with incubation of the embryos at elevated temperature, the frequency of left-right laterality defects is increased drastically in embryos with a low number of DFCs. Furthermore, we observe that these fluctuations have a large stochastic component among fish of the same genetic background. Hence, a stochastic variation in early development leads to a remarkably strong macroscopic phenotype. These fluctuations appear to be associated with maternal effects in the specification of the DFCs.
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MEDLINE
Assunto principal:
Proteínas de Peixe-Zebra
/
Embrião não Mamífero
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article