SVIP is a molecular determinant of lysosomal dynamic stability, neurodegeneration and lifespan.
Nat Commun
; 12(1): 513, 2021 01 21.
Article
em En
| MEDLINE
| ID: mdl-33479240
ABSTRACT
Missense mutations in Valosin-Containing Protein (VCP) are linked to diverse degenerative diseases including IBMPFD, amyotrophic lateral sclerosis (ALS), muscular dystrophy and Parkinson's disease. Here, we characterize a VCP-binding co-factor (SVIP) that specifically recruits VCP to lysosomes. SVIP is essential for lysosomal dynamic stability and autophagosomal-lysosomal fusion. SVIP mutations cause muscle wasting and neuromuscular degeneration while muscle-specific SVIP over-expression increases lysosomal abundance and is sufficient to extend lifespan in a context, stress-dependent manner. We also establish multiple links between SVIP and VCP-dependent disease in our Drosophila model system. A biochemical screen identifies a disease-causing VCP mutation that prevents SVIP binding. Conversely, over-expression of an SVIP mutation that prevents VCP binding is deleterious. Finally, we identify a human SVIP mutation and confirm the pathogenicity of this mutation in our Drosophila model. We propose a model for VCP disease based on the differential, co-factor-dependent recruitment of VCP to intracellular organelles.
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Base de dados:
MEDLINE
Assunto principal:
Doenças Neurodegenerativas
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Proteínas de Ligação a Fosfato
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Proteína com Valosina
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Longevidade
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Lisossomos
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Proteínas de Membrana
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Mutação
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article