Methods to Study the Transcriptome of Regulatory B Cells.
Methods Mol Biol
; 2270: 295-305, 2021.
Article
em En
| MEDLINE
| ID: mdl-33479905
ABSTRACT
Regulatory B cells do not constitute a distinct cell lineage because no unique marker or set of markers can exclusively identify neither murine nor human regulatory B cells, and efficient IL-10 production is their only known distinguishing feature. After purification of IL-10-secreting B cells, one may want to characterize them by analyzing their gene expression profile. This goal can be achieved by using different technologies RT-qPCR, microarrays, Nanostring's nCounter technology, Biomark HD are techniques that will allow you to analyze their gene expression, whether in a targeted (RT-qPCR), extended but targeted (Nanostring's nCounter technology, Biomark HD) or exhaustive (Microarray) way. Aim of this chapter is the description of these techniques in the view of their application to the study and characterization of regulatory B cells.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Perfilação da Expressão Gênica
/
Linfócitos B Reguladores
/
Transcriptoma
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article