Exact mapping of Illumina blind spots in the <i>Mycobacterium tuberculosis</i> genome reveals platform-wide and workflow-specific biases.

Modlin, Samuel J; Robinhold, Cassidy; Morrissey, Christopher; Mitchell, Scott N; Ramirez-Busby, Sarah M; Shmaya, Tal; Valafar, Faramarz

Exact mapping of Illumina blind spots in the Mycobacterium tuberculosis genome reveals platform-wide and workflow-specific biases.

Modlin, Samuel J; Robinhold, Cassidy; Morrissey, Christopher; Mitchell, Scott N; Ramirez-Busby, Sarah M; Shmaya, Tal; Valafar, Faramarz.

Afiliação

Modlin SJ; Laboratory for Pathogenesis of Clinical Drug Resistance and Persistence, School of Public Health, San Diego State University, San Diego, CA 92182, USA.
Robinhold C; Laboratory for Pathogenesis of Clinical Drug Resistance and Persistence, School of Public Health, San Diego State University, San Diego, CA 92182, USA.
Morrissey C; Laboratory for Pathogenesis of Clinical Drug Resistance and Persistence, School of Public Health, San Diego State University, San Diego, CA 92182, USA.
Mitchell SN; Laboratory for Pathogenesis of Clinical Drug Resistance and Persistence, School of Public Health, San Diego State University, San Diego, CA 92182, USA.
Ramirez-Busby SM; Laboratory for Pathogenesis of Clinical Drug Resistance and Persistence, School of Public Health, San Diego State University, San Diego, CA 92182, USA.
Shmaya T; Laboratory for Pathogenesis of Clinical Drug Resistance and Persistence, School of Public Health, San Diego State University, San Diego, CA 92182, USA.
Valafar F; Laboratory for Pathogenesis of Clinical Drug Resistance and Persistence, School of Public Health, San Diego State University, San Diego, CA 92182, USA.

Microb Genom ; 7(3)2021 03.

Article em En | MEDLINE | ID: mdl-33502304

Assuntos

Sequenciamento de Nucleotídeos em Larga Escala/normas; Mycobacterium tuberculosis/genética; Tuberculose/microbiologia; Viés; Biblioteca Gênica; Genoma Bacteriano; Sequenciamento de Nucleotídeos em Larga Escala/métodos; Humanos; Mycobacterium tuberculosis/classificação; Mycobacterium tuberculosis/isolamento & purificação; Sequenciamento Completo do Genoma/métodos; Sequenciamento Completo do Genoma/normas; Fluxo de Trabalho

Palavras-chave

Illumina; blind spots; coverage bias; homopolymers; sequencing; tuberculosis

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tuberculose / Sequenciamento de Nucleotídeos em Larga Escala / Mycobacterium tuberculosis Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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