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Non-cell-autonomous migration of RasV12-transformed cells towards the basal side of surrounding normal cells.
Jebri, Imen; Tsujita, Kazuya; Fujita, Yasuyuki; Itoh, Toshiki.
Afiliação
  • Jebri I; Division of Membrane Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, 650-0017, Japan.
  • Tsujita K; Division of Membrane Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, 650-0017, Japan; Biosignal Research Center, Kobe University, Kobe, 657-8501, Japan.
  • Fujita Y; Division of Molecular Oncology, Institute for Genetic Medicine, Hokkaido University Graduate School of Chemical Sciences and Engineering, Sapporo, 060-0815, Japan; Division of Molecular Oncology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8501, Japan.
  • Itoh T; Division of Membrane Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, 650-0017, Japan; Biosignal Research Center, Kobe University, Kobe, 657-8501, Japan. Electronic address: titoh@people.kobe-u.ac.jp.
Biochem Biophys Res Commun ; 543: 15-22, 2021 03 05.
Article em En | MEDLINE | ID: mdl-33503542
Oncogenic transformation enables cells to behave differently from their neighboring normal cells. Both cancer and normal cells recognize each other, often promoting the extrusion of the former from the epithelial cell layer. Here, we show that RasV12-transformed normal rat kidney 52E (NRK-52E) cells are extruded towards the basal side of the surrounding normal cells, which is concomitant with enhanced motility. The active migration of the basally extruded RasV12 cells is observed when surrounded by normal cells, indicating a non-cell-autonomous mechanism. Furthermore, specific inhibitor treatment and knockdown experiments elucidate the roles of PI3K and myosin IIA in the basal extrusion of Ras cells. Our findings reveal a new aspect of cancer cell invasion mediated by functional interactions with surrounding non-transformed cells.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Valina / Proteína Oncogênica p21(ras) / Fosfatidilinositol 3-Quinases / Miosina não Muscular Tipo IIA / Mutação / Neoplasias Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Valina / Proteína Oncogênica p21(ras) / Fosfatidilinositol 3-Quinases / Miosina não Muscular Tipo IIA / Mutação / Neoplasias Idioma: En Ano de publicação: 2021 Tipo de documento: Article