Proteomic Analysis Uncovers Measles Virus Protein C Interaction With p65-iASPP Protein Complex.
Mol Cell Proteomics
; 20: 100049, 2021.
Article
em En
| MEDLINE
| ID: mdl-33515806
ABSTRACT
Viruses manipulate the central machineries of host cells to their advantage. They prevent host cell antiviral responses to create a favorable environment for their survival and propagation. Measles virus (MV) encodes two nonstructural proteins MV-V and MV-C known to counteract the host interferon response and to regulate cell death pathways. Several molecular mechanisms underlining MV-V regulation of innate immunity and cell death pathways have been proposed, whereas MV-C host-interacting proteins are less studied. We suggest that some cellular factors that are controlled by MV-C protein during viral replication could be components of innate immunity and the cell death pathways. To determine which host factors are targeted by MV-C, we captured both direct and indirect host-interacting proteins of MV-C protein. For this, we used a strategy based on recombinant viruses expressing tagged viral proteins followed by affinity purification and a bottom-up mass spectrometry analysis. From the list of host proteins specifically interacting with MV-C protein in different cell lines, we selected the host targets that belong to immunity and cell death pathways for further validation. Direct protein interaction partners of MV-C were determined by applying protein complementation assay and the bioluminescence resonance energy transfer approach. As a result, we found that MV-C protein specifically interacts with p65-iASPP protein complex that controls both cell death and innate immunity pathways and evaluated the significance of these host factors on virus replication.
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MEDLINE
Assunto principal:
Proteínas Repressoras
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Proteínas não Estruturais Virais
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Peptídeos e Proteínas de Sinalização Intracelular
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Fator de Transcrição RelA
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article