Global, cell non-autonomous gene regulation drives individual lifespan among isogenic C. elegans.
Elife
; 102021 02 01.
Article
em En
| MEDLINE
| ID: mdl-33522488
ABSTRACT
Across species, lifespan is highly variable among individuals within a population. Even genetically identical Caenorhabditis elegans reared in homogeneous environments are as variable in lifespan as outbred human populations. We hypothesized that persistent inter-individual differences in expression of key regulatory genes drives this lifespan variability. As a test, we examined the relationship between future lifespan and the expression of 22 microRNA promoterGFP constructs. Surprisingly, expression of nearly half of these reporters, well before death, could effectively predict lifespan. This indicates that prospectively long- vs. short-lived individuals have highly divergent patterns of transgene expression and transcriptional regulation. The gene-regulatory processes reported on by two of the most lifespan-predictive transgenes do not require DAF-16, the FOXO transcription factor that is a principal effector of insulin/insulin-like growth factor (IGF-1) signaling. Last, we demonstrate a hierarchy of redundancy in lifespan-predictive ability among three transgenes expressed in distinct tissues, suggesting that they collectively report on an organism-wide, cell non-autonomous process that acts to set each individual's lifespan.
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Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
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Caenorhabditis elegans
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MicroRNAs
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Longevidade
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article