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Silk-based hybrid microfibrous mats as guided bone regeneration membranes.
Wu, Mi; Han, Zhengyi; Liu, Wen; Yao, Jinrong; Zhao, Bingjiao; Shao, Zhengzhong; Chen, Xin.
Afiliação
  • Wu M; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital, Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China. chenx@fudan.edu.cn.
  • Han Z; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital, Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China. chenx@fudan.edu.cn.
  • Liu W; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital, Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China. chenx@fudan.edu.cn.
  • Yao J; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital, Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China. chenx@fudan.edu.cn.
  • Zhao B; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital, Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China. chenx@fudan.edu.cn.
  • Shao Z; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital, Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China. chenx@fudan.edu.cn.
  • Chen X; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital, Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China. chenx@fudan.edu.cn.
J Mater Chem B ; 9(8): 2025-2032, 2021 03 04.
Article em En | MEDLINE | ID: mdl-33555002
ABSTRACT
The usage of a guided bone regeneration (GBR) membrane that prevents the ingrowth of fibroblast cells and enhances the regeneration rate is an effective strategy for bone regeneration therapy. Herein, LAPONITE® (LAP) nanoplatelets, a bioactive clay with good osteoinductivity, were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning. The as-prepared RSF-LAP hybrid microfibrous mats had an interconnected structure with pore size significantly smaller than that of the fibroblast cells, leading to an effective prevention of fibroblast cell ingrowth into the defect sites. As per the water contact angle measurements, the incorporation of LAP significantly improved the hydrophilicity of the RSF microfibrous mats. The in vitro cell experiment results show that the RSF-LAP microfibrous mats exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF microfibrous mats. Moreover, the RSF-LAP microfibrous mats promoted osteogenic differentiation by upregulating alkaline phosphatase (ALP) activity and osteo-specific gene expression. Therefore, the results suggest that this easily fabricated LAP-incorporated RSF microfibrous mat has great potential to be a promising biomaterial for GBR applications.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Materiais Biocompatíveis / Regeneração Óssea / Silicatos / Seda / Membranas Artificiais Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
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XML
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Materiais Biocompatíveis / Regeneração Óssea / Silicatos / Seda / Membranas Artificiais Idioma: En Ano de publicação: 2021 Tipo de documento: Article