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Where are G-quadruplexes located in the human transcriptome?
Vannutelli, Anaïs; Belhamiti, Sarah; Garant, Jean-Michel; Ouangraoua, Aida; Perreault, Jean-Pierre.
Afiliação
  • Vannutelli A; Department of Computer Science, Faculté des sciences, Université de Sherbrooke, QC J1K 2R1, Canada.
  • Belhamiti S; Department of Biochemistry and Functional Genomics, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, QC J1E 4K8, Canada.
  • Garant JM; Department of Computer Science, Faculté des sciences, Université de Sherbrooke, QC J1K 2R1, Canada.
  • Ouangraoua A; Department of Biochemistry and Functional Genomics, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, QC J1E 4K8, Canada.
  • Perreault JP; Department of Biochemistry and Functional Genomics, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, QC J1E 4K8, Canada.
NAR Genom Bioinform ; 2(2): lqaa035, 2020 Jun.
Article em En | MEDLINE | ID: mdl-33575590
It has been demonstrated that RNA G-quadruplexes (G4) are structural motifs present in transcriptomes and play important regulatory roles in several post-transcriptional mechanisms. However, the full picture of RNA G4 locations and the extent of their implication remain elusive. Solely computational prediction analysis of the whole transcriptome may reveal all potential G4, since experimental identifications are always limited to specific conditions or specific cell lines. The present study reports the first in-depth computational prediction of potential G4 region across the complete human transcriptome. Although using a relatively stringent approach based on three prediction scores that accounts for the composition of G4 sequences, the composition of their neighboring sequences, and the various forms of G4, over 1.1 million of potential G4 (pG4) were predicted. The abundance of G4 was computationally confirmed in both 5' and 3'UTR as well as splicing junction of mRNA, appreciate for the first time in the long ncRNA, while almost absent of most of the small ncRNA families. The present results constitute an important step toward a full understanding of the roles of G4 in post-transcriptional mechanisms.

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article