Dioclea Altissima Seed Lectin (DAL) Prevents Anxiety-like Behavioral Responses in Adult Zebrafish (Danio Rerio): Involvement of GABAergic and 5-HT Systems.
CNS Neurol Disord Drug Targets
; 21(1): 95-103, 2022.
Article
em En
| MEDLINE
| ID: mdl-33583388
ABSTRACT
BACKGROUND:
Plant lectins have shown promising neuropharmacological activities in animal models.OBJECTIVE:
This study evaluated the effect of Dioclea altissima seed lectin (DAL) on adult zebrafish behavior.METHOD:
Zebrafish (n=6/group) were treated (i.p.; 20 µL) with DAL (0.025; 0.05 or 0.1 mg/mL), vehicle or diazepam (DZP) and submitted to several tests (open field, light/dark preference or novel tank). Flumazenil, pizotifen or granisetron were administered 15 min before DAL (0.05 mg/mL), and the animals were evaluated on light/dark preference test. It was also verified whether the DAL effect depended on its structural integrity and ability to interact with carbohydrates.RESULTS:
DAL decreased the locomotor activity of adult zebrafish (0.025; 0.05 or 0.1 mg/mL), increased the time spent in the upper region of the aquarium (0.025 mg/mL), and decreased the latency time of adult zebrafish to enter the upper region on the novel tank test. DAL (0.05 mg/mL) also increased their permanence in the light zone of the light/dark preference test. The effect of DAL was dependent on carbohydrate interaction and protein structure integrity and was prevented by pizotifen, granizetron and flumazenil.CONCLUSION:
DAL was found to have an anxiolytic-like effect mediated by the 5-HT and GABAergic receptors.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Ansiedade
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Comportamento Animal
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Peixe-Zebra
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Dioclea
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Lectinas
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article