Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster.
Development
; 148(6)2021 03 16.
Article
em En
| MEDLINE
| ID: mdl-33593816
ABSTRACT
Cellular development and function rely on highly dynamic molecular interactions among proteins distributed in all cell compartments. Analysis of these interactions has been one of the main topics in cellular and developmental research, and has been mostly achieved by the manipulation of proteins of interest (POIs) at the genetic level. Although genetic strategies have significantly contributed to our current understanding, targeting specific interactions of POIs in a time- and space-controlled manner or analysing the role of POIs in dynamic cellular processes, such as cell migration or cell division, would benefit from more-direct approaches. The recent development of specific protein binders, which can be expressed and function intracellularly, along with advancement in synthetic biology, have contributed to the creation of a new toolbox for direct protein manipulations. Here, we have selected a number of short-tag epitopes for which protein binders from different scaffolds have been generated and showed that single copies of these tags allowed efficient POI binding and manipulation in living cells. Using Drosophila, we also find that single short tags can be used for POI manipulation in vivo.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Proteínas
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Drosophila melanogaster
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Epitopos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article