Magnetic ligand fishing using immobilized DPP-IV for identification of antidiabetic ligands in lingonberry extract.
PLoS One
; 16(2): e0247329, 2021.
Article
em En
| MEDLINE
| ID: mdl-33617581
ABSTRACT
In this work, a new magnetic ligand fishing probe for discovery of DPP-IV inhibitory ligands was developed and it was tested as a proof of concept on the fruit extract of Vaccinium vitis-idaea (lingonberry). The ligands were shown to have appreciable dipeptidyl peptidase IV (DPP-IV) inhibitory activity (IC50 31.8 µg mL-1).) Inhibition of DPP-IV is a well-known therapeutic approach for management of type 2 diabetes (T2D). DPP-IV was successfully immobilized onto magnetic beads and was shown to retain its catalytic activity and selectivity over a model mixture. A total of four ligands were successfully fished out and identified as cyanidin-3-galactoside (2), cyanidin-3-arabinoside (3), proanthocynidin A (4), and 10-carboxyl-pyranopeonidin 3-O-(6â³-O-p-coumaroyl)-glucoside (5) using HPLC/HRMS.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Dipeptidil Peptidase 4
/
Vaccinium vitis-Idaea
/
Inibidores da Dipeptidil Peptidase IV
/
Hipoglicemiantes
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article