Circ_0000003 regulates glutamine metabolism and tumor progression of tongue squamous cell carcinoma via the miR3303p/GLS axis.
Oncol Rep
; 45(4)2021 04.
Article
em En
| MEDLINE
| ID: mdl-33649795
ABSTRACT
Various circular RNAs (circRNAs) have been shown to exert vital functions in tongue squamous cell carcinoma (TSCC). However, their roles in TSCC progression remain to be elucidated. This research aimed to investigate the role and mechanism of hsa_circ_0000003 (circ_0000003) in TSCC progression. Here, we found that circ_0000003 expression was upregulated in TSCC tissues and cell lines, and high circ_0000003 expression was correlated with advanced TNM stage, increased tumor size and poor patient survival. Circ_0000003 was revealed to facilitate cell proliferation, migration and invasion of TSCC cells. Mechanistically, we found that circ_0000003 acted as a competing endogenous RNA (ceRNA) that sponged miR3303p, thereby elevating glutaminase (GLS) expression. Accordingly, cell invasion, migration, glutamine consumption, αketoglutarate (αKG) production and ATP production were significantly decreased by circ_0000003 knockdown in TSCC cells, and these effects were reversed by miR3303p inhibition. In conclusion, circ_0000003 facilitates TSCC cell proliferation, migration, invasion and glutamine catabolism by regulating the miR3303p/GLS pathway.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Língua
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MicroRNAs
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Carcinoma de Células Escamosas de Cabeça e Pescoço
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RNA Circular
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Glutaminase
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article