lncRNA transcription induces meiotic recombination through chromatin remodelling in fission yeast.
Commun Biol
; 4(1): 295, 2021 03 05.
Article
em En
| MEDLINE
| ID: mdl-33674718
ABSTRACT
Noncoding RNAs (ncRNAs) are involved in various biological processes, including gene expression, development, and disease. Here, we identify a novel consensus sequence of a cis-element involved in long ncRNA (lncRNA) transcription and demonstrate that lncRNA transcription from this cis-element activates meiotic recombination via chromatin remodeling. In the fission yeast fbp1 gene, glucose starvation induces a series of promoter-associated lncRNAs, referred to as metabolic-stress-induced lncRNAs (mlonRNAs), which contribute to chromatin remodeling and fbp1 activation. Translocation of the cis-element required for mlonRNA into a well-characterized meiotic recombination hotspot, ade6-M26, further stimulates transcription and meiotic recombination via local chromatin remodeling. The consensus sequence of this cis-element (mlon-box) overlaps with meiotic recombination sites in the fission yeast genome. At one such site, the SPBC24C6.09c upstream region, meiotic double-strand break (DSB) formation is induced in an mlon-box-dependent manner. Therefore, mlonRNA transcription plays a universal role in chromatin remodeling and the regulation of transcription and recombination.
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Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
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Transcrição Gênica
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RNA Fúngico
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Montagem e Desmontagem da Cromatina
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Reparo de DNA por Recombinação
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RNA Longo não Codificante
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Meiose
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article