Effects of Recombinant Angiogenin on Collagen Fiber Formation and Angiogenesis in the Dermis of Wistar Rats.

Yurina, Natalia V; Ageeva, Tatiana A; Goryachkin, Aleksandr M; Varaksin, Nikolay A; Ryabicheva, Tatiana G; Ostanin, Alexandr A; Chernykh, Elena R; Romashchenko, Alexander V; Proskurina, Anastasia S; Bogachev, Sergey; Purtov, Alexey V

Yurina, Natalia V; Ageeva, Tatiana A; Goryachkin, Aleksandr M; Varaksin, Nikolay A; Ryabicheva, Tatiana G; Ostanin, Alexandr A; Chernykh, Elena R; Romashchenko, Alexander V; Proskurina, Anastasia S; Bogachev, Sergey; Purtov, Alexey V.

Afiliação

Yurina NV; Autonomous Non-Profit Organization "Regional Center for High Medical Technologies", Novosibirsk, Russia.
Ageeva TA; Novosibirsk State Medical University, Novosibirsk, Russia.
Goryachkin AM; Novosibirsk State Medical University, Novosibirsk, Russia.
Varaksin NA; JSC "Vector-Best", Novosibirsk, Russia.
Ryabicheva TG; JSC "Vector-Best", Novosibirsk, Russia.
Ostanin AA; Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russia.
Chernykh ER; Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russia.
Romashchenko AV; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Proskurina AS; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Bogachev S; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Purtov AV; LLC "Angiopharm Laboratory", Novosibirsk, Russia.

Clin Cosmet Investig Dermatol ; 14: 187-196, 2021.

Article em En | MEDLINE | ID: mdl-33679135

ABSTRACT

ABSTRACT

PURPOSE:

The purpose of this study was to assess the capability of recombinant angiogenin isolated from Pichia pastoris yeasts to stimulate regenerative processes in the dermis of experimental animals. PATIENTS AND

METHODS:

Wistar rats were administered with recombinant angiogenin intracutaneously. Morphological examination of the skin and the assessment of the proliferative activity of the epidermal cells were carried out. Additionally, cytokine production by human whole blood cells exposed to angiogenin was analyzed ex vivo.

RESULTS:

Administration of angiogenin stimulates collagen fiber formation and angiogenesis. This stimulation is tightly associated with an increase in the number of fibroblasts, an increased numerical density of dermal blood vessels and an increased density of collagen fibers; also, it activates the proliferation of basal cells. Angiogenin induces the production of MCP, IL-8, IL-6, IL-1ß, TNF-α, IL-10, TGF-ß, and VEGF by blood cells.