Pseudotargeted Lipidomics Strategy Enabling Comprehensive Profiling and Precise Lipid Structural Elucidation of Polyunsaturated Lipid-Rich Echium Oil.
J Agric Food Chem
; 69(32): 9012-9024, 2021 Aug 18.
Article
em En
| MEDLINE
| ID: mdl-33683118
ABSTRACT
Echium oil has great nutritional value as a result of its high content of α-linolenic acid (ALA, 183ω-3) and stearidonic acid (SDA, 184ω-3). However, the comprehensive lipid profiling and exact structural characterization of bioactive polyunsaturated lipids in echium oil have not yet been obtained. In this study, we developed a novel pseudotargeted lipidomics strategy for comprehensive profiling and lipid structural elucidation of polyunsaturated lipid-rich echium oil. Our approach integrated untargeted lipidomics analysis with a targeted lipidomics strategy based on Paternò-Büchi (PB)-tandem mass spectrometry (MS/MS) using 2-acetylpyridine (2-AP) as the reaction reagent, allowing for high-coverage lipid profiling and simultaneous determination of CâC locations in triacylglycerols (TGs), diacylglycerols (DGs), free fatty acids (FFAs), and sterol esters (SEs) in echium oil. A total of 209 lipid species were profiled, among which 162 unsaturated lipids were identified with CâC location assignment and 42 groups of ω-3 and ω-6 CâC location isomers were discovered. In addition, relative isomer ratios of certain groups of lipid CâC location isomers were revealed. This pseudotargeted lipidomics strategy described in this study is expected to provide new insight into structural characterization of distinctive bioactive lipids in food.
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Graxos Ômega-3
/
Echium
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article