Aptamer-based proximity labeling guides covalent RNA modification.
Chem Commun (Camb)
; 57(28): 3480-3483, 2021 Apr 11.
Article
em En
| MEDLINE
| ID: mdl-33688891
ABSTRACT
We describe the development of a proximity-induced bio-orthogonal inverse electron demand Diels-Alder reaction that exploits the high-affinity interaction between a dienophile-modified RhoBAST aptamer and its tetramethyl rhodamine methyltetrazine substrate. We applied this concept for covalent RNA labeling in proof-of-principle experiments.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
RNA
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Aptâmeros de Nucleotídeos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article