Evaluating Melanoma Viability and Proliferation in 3D Microenvironments.
Methods Mol Biol
; 2265: 155-171, 2021.
Article
em En
| MEDLINE
| ID: mdl-33704713
ABSTRACT
Researchers often aim to incorporate microenvironmental variables such as the dimensionality and composition of the extracellular matrix into their cell-based assays. A technical challenge created by introduction of these variables is quantification of single-cell measurements and control of environmental reproducibility. Here, we detail a methodology to quantify viability and proliferation of melanoma cells in 3D collagen-based culture platforms by automated microscopy and 3D image analysis to yield robust, high-throughput results of single-cell responses to drug treatment.
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Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
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Técnicas de Cultura de Células
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Inibidores de Proteínas Quinases
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Proliferação de Células
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Análise de Célula Única
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Melanoma
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Antineoplásicos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article