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Unbiased Microbiome and Metabolomic Profiling of Fecal Samples from Patients with Melanoma.
Bui, Ashley; Choi, Yongbin; Frankel, Arthur E; Koh, Andrew Y.
Afiliação
  • Bui A; Department of Pediatrics, University of Texas Southwestern, Dallas, TX, USA.
  • Choi Y; Department of Pediatrics, University of Texas Southwestern, Dallas, TX, USA.
  • Frankel AE; Department of Internal Medicine, West Palm Beach Veterans Administration Medical Center, West Palm Beach, FL, USA.
  • Koh AY; Department of Pediatrics, University of Texas Southwestern, Dallas, TX, USA. andrew.koh@utsouthwestern.edu.
Methods Mol Biol ; 2265: 461-474, 2021.
Article em En | MEDLINE | ID: mdl-33704734
Gut microbiota influence and modulate host immune responses. In preclinical cancer models, mice lacking gut microbiota have a markedly diminished response to immune checkpoint inhibitor therapy. Further, in melanoma patients, specific commensal gut microbiota have been associated with a positive clinical response to immunotherapy. In order to study the gut microbiome and metabolome, we have developed methods for fecal sample collection and processing, microbiome and metabolome profiling, and bioinformatic analysis. This protocol will be a useful tool for interrogating the taxonomic composition and functional output of a melanoma patient's gut microbiome.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Metaboloma / Metabolômica / Fezes / Microbioma Gastrointestinal / Melanoma Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Metaboloma / Metabolômica / Fezes / Microbioma Gastrointestinal / Melanoma Idioma: En Ano de publicação: 2021 Tipo de documento: Article