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Evaluation of molecular techniques to visceral leishmaniasis detection in asymptomatic patients: a systematic review.
Virginia Batista Vieira, Amanda; Farias, Pablo Cantalice Santos; Silva Nunes Bezerra, Gilberto; Xavier, Amanda Tavares; Sebastião Da Costa Lima Júnior, Manoel; Silva, Elis Dionísio Da; Barbosa Júnior, Walter Lins; Medeiros, Zulma Maria.
Afiliação
  • Virginia Batista Vieira A; Programa De Pós-Graduação Em Ciências Da Saúde, Universidade De Pernambuco (UPE), Recife, Brasil.
  • Farias PCS; Departamento De Parasitologia, Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (FIOCRUZ), Recife, Brasil.
  • Silva Nunes Bezerra G; Departamento De Parasitologia, Universidade Federal De Pernambuco (UFPE), Recife, Brasil.
  • Xavier AT; Programa De Pós-Graduação Em Ciências Da Saúde, Universidade De Pernambuco (UPE), Recife, Brasil.
  • Sebastião Da Costa Lima Júnior M; Departamento De Parasitologia, Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (FIOCRUZ), Recife, Brasil.
  • Silva EDD; Programa De Pós-Graduação Em Ciências Da Saúde, Universidade De Pernambuco (UPE), Recife, Brasil.
  • Barbosa Júnior WL; Departamento De Parasitologia, Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (FIOCRUZ), Recife, Brasil.
  • Medeiros ZM; Departamento De Imunologia, Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (FIOCRUZ), Recife, Brasil.
Expert Rev Mol Diagn ; 21(5): 493-504, 2021 May.
Article em En | MEDLINE | ID: mdl-33719847
ABSTRACT

INTRODUCTION:

Visceral leishmaniasis (VL) is a systemic and neglected parasitic disease. Its main symptoms are fever, splenomegaly with or without hepatomegaly, and anemia, however, most individuals remain asymptomatic. Due to the lack of a gold standard and the limitations of current diagnostic techniques, where parasitology is ethically unfeasible for individuals without symptoms and serological tests do not differentiate between past and present disease, molecular methodologies are the most suitable. AREAS COVERED We performed a systematic review analyzing the molecular techniques based on PCR used, so far, to detect asymptomatic cases of VL in humans. Structured searches were carried out on PubMed, LILACS, Scopus, and Web of Science databases without time and language restrictions. Two reviewers evaluated the studies, performed data extraction, and quality assessment by assigning scores. EXPERT OPINION qPCR using RNA targets can be used in the diagnosis of asymptomatic cases of human VL, due to its characteristics. We recommend further studies to analyze the methodology, mainly observing the use of different rRNA targets. Therefore, we hope that this technique contributed to the construction of public policies that address the diagnosis and handling of asymptomatic patients.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Leishmaniose Visceral Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Leishmaniose Visceral Idioma: En Ano de publicação: 2021 Tipo de documento: Article