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Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients.
Cardoso, Chandra Chiappin; Matiollo, Camila; Pereira, Carolina Hilgert Jacobsen; Fonseca, Janaina Santana; Alves, Helder Emmanuel Leite; da Silva, Otavio Marcos; de Souza Menegassi, Vivian; Dos Santos, Claudia Regina; de Moraes, Ana Carolina Rabello; de Lucca Schiavon, Leonardo; Santos-Silva, Maria Claudia.
Afiliação
  • Cardoso CC; Division of Clinical Analysis, Flow Cytometry Service, Health Sciences Center, University Hospital of the Federal University of Santa Catarina, Florianópolis, SC, 88040-900, Brazil. chandraccardoso@gmail.com.
  • Matiollo C; Postgraduate Program in Pharmacy of the Federal University of Santa Catarina, Florianópolis, SC, Brazil. chandraccardoso@gmail.com.
  • Pereira CHJ; Postgraduate Program in Medical Sciences of the Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Fonseca JS; Postgraduate Program in Pharmacy of the Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Alves HEL; Division of Gastroenterology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • da Silva OM; Division of Gastroenterology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • de Souza Menegassi V; Division of Gastroenterology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Dos Santos CR; Division of Gastroenterology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • de Moraes ACR; Postgraduate Program in Pharmacy of the Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • de Lucca Schiavon L; Clinical Analysis Department, Health Sciences Center, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Santos-Silva MC; Postgraduate Program in Pharmacy of the Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Sci Rep ; 11(1): 5923, 2021 03 15.
Article em En | MEDLINE | ID: mdl-33723292
Liver cirrhosis is often complicated by an immunological imbalance known as cirrhosis-associated immune dysfunction. This study aimed to investigate disturbances in circulating monocytes and dendritic cells in patients with acute decompensation (AD) of cirrhosis. The sample included 39 adult cirrhotic patients hospitalized for AD, 29 patients with stable cirrhosis (SC), and 30 healthy controls (CTR). Flow cytometry was used to analyze monocyte and dendritic cell subsets in whole blood and quantify cytokines in plasma samples. Cirrhotic groups showed higher frequencies of intermediate monocytes (iMo) than CTR. AD patients had lower percentages of nonclassical monocytes than CTR and SC. Cirrhotic patients had a profound reduction in absolute and relative dendritic cell numbers compared with CTR and showed higher plasmacytoid/classical dendritic cell ratios. Increased plasma levels of IL-6, IL-10, and IL-17A, elevated percentages of CD62L+ monocytes, and reduced HLA-DR expression on classical monocytes (cMo) were also observed in cirrhotic patients. Patients with more advanced liver disease showed increased cMo and reduced tissue macrophages (TiMas) frequencies. It was found that cMo percentages greater than 90.0% within the monocyte compartment and iMo and TiMas percentages lower than 5.7% and 8.6%, respectively, were associated with increased 90-day mortality. Monocytes and dendritic cells are deeply altered in cirrhotic patients, and subset profiles differ between stable and advanced liver disease. High cMo and low TiMas frequencies may be useful biomarkers of disease severity and mortality in liver cirrhosis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Dendríticas / Monócitos / Cirrose Hepática Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Dendríticas / Monócitos / Cirrose Hepática Idioma: En Ano de publicação: 2021 Tipo de documento: Article