N-acetylcysteine delivery with silica nanoparticles into 3T3-L1 adipocytes.
Ther Deliv
; 12(4): 287-296, 2021 04.
Article
em En
| MEDLINE
| ID: mdl-33726506
ABSTRACT
Background:
The addition of 5 mM N-acetylcysteine (NAC) to 3T3-L1 adipocytes culture inhibits the accumulation of triglycerides (Tg) by 50%, but after 48 h uptake was only 16% of total NAC available. Based on these results, the aim of this study is to increase the NAC cellular uptake by encapsulating it in silica nanoparticles (NPs). Materials &methods:
Silica NPs, 20 ± 4.5 nm in size, were developed, with an inner cavity loaded with 5 mM NAC. At 48 h after treatment, there was a dose-dependent cytotoxic effect. We attempted to reduce the cytotoxicity of silica NPs by coating them with bovine serum albumin.Results:
While we obtained nontoxic bovine serum albumin coated NPs, their effect on Tg cellular accumulation was also reduced.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Acetilcisteína
/
Nanopartículas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article