Direct ligand screening against membrane proteins on live cells enabled by DNA-programmed affinity labelling.
Chem Commun (Camb)
; 57(31): 3769-3772, 2021 Apr 21.
Article
em En
| MEDLINE
| ID: mdl-33730131
ABSTRACT
Membrane proteins are important drug targets; however, ligand discovery for membrane proteins is highly challenging due to their hydrophobic nature. We show that membrane proteins may be specifically labelled with a DNA tag by DNA-programmed affinity labelling (DPAL), thereby enabling the screening of chemical compounds against membrane proteins directly on live cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Marcadores de Afinidade
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Bibliotecas de Moléculas Pequenas
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Proteínas de Membrana
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article