Integrated proteomic sample preparation with combination of on-line high-abundance protein depletion, denaturation, reduction, desalting and digestion to achieve high throughput plasma proteome quantification.

Li, Yilan; Yuan, Huiming; Dai, Zhongpeng; Zhang, Weijie; Zhang, Xiaodan; Zhao, Baofeng; Liang, Zhen; Zhang, Lihua; Zhang, Yukui

Li, Yilan; Yuan, Huiming; Dai, Zhongpeng; Zhang, Weijie; Zhang, Xiaodan; Zhao, Baofeng; Liang, Zhen; Zhang, Lihua; Zhang, Yukui.

Afiliação

Li Y; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Yuan H; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China. Electronic address: huimingyuan@dicp.ac.cn.
Dai Z; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.
Zhang W; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Zhang X; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.
Zhao B; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.
Liang Z; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.
Zhang L; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China. Electronic address: lihuazhang@dicp.ac.cn.
Zhang Y; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116023, China.

Anal Chim Acta ; 1154: 338343, 2021 Apr 15.

Article em En | MEDLINE | ID: mdl-33736814

Assuntos

Proteoma; Proteômica; Proteínas Sanguíneas; Cromatografia Líquida; Digestão; Humanos; Reprodutibilidade dos Testes; Espectrometria de Massas em Tandem

Palavras-chave

High-abundance protein depletion; Integrated plasma sample preparation; Label-free quantification; On-line middle and low-abundance proteins treatment

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteoma / Proteômica Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteoma / Proteômica Idioma: En Ano de publicação: 2021 Tipo de documento: Article