Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State.
Methods Mol Biol
; 2280: 179-187, 2021.
Article
em En
| MEDLINE
| ID: mdl-33751435
ABSTRACT
To perform their action, flavoproteins usually interact with a variety of low molecular weight partners, including electron transporters, yielding transient complexes whose tightness is often controlled by the redox state of the bound flavin cofactor. As a case study, here we describe the quantitative analysis of the redox-dependent interaction of the mammalian apoptosis inducing factor (AIF) with its NAD+ ligand. In particular, we report a protocol for the spectrophotometric titration of AIF in its reduced state under anaerobic conditions with NAD+, in order to determine the dissociation constant of the resulting complex.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Escherichia coli
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Fator de Indução de Apoptose
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NAD
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article