Filopodium-derived vesicles produced by MIM enhance the migration of recipient cells.
Dev Cell
; 56(6): 842-859.e8, 2021 03 22.
Article
em En
| MEDLINE
| ID: mdl-33756122
ABSTRACT
Extracellular vesicles (EVs) are classified as large EVs (l-EVs, or microvesicles) and small EVs (s-EVs, or exosomes). S-EVs are thought to be generated from endosomes through a process that mainly depends on the ESCRT protein complex, including ALG-2 interacting protein X (ALIX). However, the mechanisms of l-EV generation from the plasma membrane have not been identified. Membrane curvatures are generated by the bin-amphiphysin-rvs (BAR) family proteins, among which the inverse BAR (I-BAR) proteins are involved in filopodial protrusions. Here, we show that the I-BAR proteins, including missing in metastasis (MIM), generate l-EVs by scission of filopodia. Interestingly, MIM-containing l-EV production was promoted by in vivo equivalent external forces and by the suppression of ALIX, suggesting an alternative mechanism of vesicle formation to s-EVs. The MIM-dependent l-EVs contained lysophospholipids and proteins, including IRS4 and Rac1, which stimulated the migration of recipient cells through lamellipodia formation. Thus, these filopodia-dependent l-EVs, which we named as filopodia-derived vesicles (FDVs), modify cellular behavior.
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Base de dados:
MEDLINE
Assunto principal:
Pseudópodes
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Proteínas de Ligação ao Cálcio
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Movimento Celular
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Proteínas de Ciclo Celular
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Endocitose
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Exossomos
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Complexos Endossomais de Distribuição Requeridos para Transporte
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Vesículas Extracelulares
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Proteínas dos Microfilamentos
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Proteínas de Neoplasias
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article