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Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike.
Tong, Pei; Gautam, Avneesh; Windsor, Ian; Travers, Meghan; Chen, Yuezhou; Garcia, Nicholas; Whiteman, Noah B; McKay, Lindsay G A; Lelis, Felipe J N; Habibi, Shaghayegh; Cai, Yongfei; Rennick, Linda J; Duprex, W Paul; McCarthy, Kevin R; Lavine, Christy L; Zuo, Teng; Lin, Junrui; Zuiani, Adam; Feldman, Jared; MacDonald, Elizabeth A; Hauser, Blake M; Griffths, Anthony; Seaman, Michael S; Schmidt, Aaron G; Chen, Bing; Neuberg, Donna; Bajic, Goran; Harrison, Stephen C; Wesemann, Duane R.
Afiliação
  • Tong P; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Gautam A; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Windsor I; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
  • Travers M; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Chen Y; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Garcia N; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Whiteman NB; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • McKay LGA; Department of Microbiology, Boston University School of Medicine, Boston, MA 02115, USA.
  • Lelis FJN; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA.
  • Habibi S; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Cai Y; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Rennick LJ; Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.
  • Duprex WP; The Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261.
  • McCarthy KR; The Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh PA.
  • Lavine CL; The Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261.
  • Zuo T; The Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh PA.
  • Lin J; The Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261.
  • Zuiani A; The Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh PA.
  • Feldman J; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.
  • MacDonald EA; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Hauser BM; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Griffths A; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Seaman MS; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.
  • Schmidt AG; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
  • Chen B; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.
  • Neuberg D; Department of Microbiology, Boston University School of Medicine, Boston, MA 02115, USA.
  • Bajic G; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA.
  • Harrison SC; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.
  • Wesemann DR; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.
bioRxiv ; 2021 Mar 10.
Article em En | MEDLINE | ID: mdl-33758863
ABSTRACT
Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory B cell receptor-encoded monoclonal antibodies (mAbs) from 19 COVID-19 convalescent subjects against SARS-CoV-2 spike (S) and found 7 major mAb competition groups against epitopes recurrently targeted across individuals. Inclusion of published and newly determined structures of mAb-S complexes identified corresponding epitopic regions. Group assignment correlated with cross-CoV-reactivity breadth, neutralization potency, and convergent antibody signatures. mAbs that competed for binding the original S isolate bound differentially to S variants, suggesting the protective importance of otherwise-redundant recognition. The results furnish a global atlas of the S-specific memory B cell repertoire and illustrate properties conferring robustness against emerging SARS-CoV-2 variants.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article