Sequential Changes of Total Adenine Nucleotide and Adenylic-Acid Energy Charge in Muscles of Rats after Death.
Fa Yi Xue Za Zhi
; 37(1): 7-10, 2021 Feb.
Article
em En, Zh
| MEDLINE
| ID: mdl-33780177
ABSTRACT
ABSTRACT Objective To determine the purine adenylate [adenosine triphosphate ï¼ATPï¼, adenosine diphosphate ï¼ADPï¼, adenosine monophosphate ï¼AMPï¼] content in the muscles of both hind limbs of rats at different postmortem interval ï¼PMIï¼, calculate the changes in the total adenine nucleotide ï¼TANï¼ content and the adenylic-acid energy charge ï¼AECï¼, and explore their relationship with PMI. Methods Healthy rats were sacrificed by cervical dislocation and kept at 20 â. The muscles of their hind limbs were extracted at 0, 24, 48, 72, 96, 120, 144, and 168 h after death. Reversed-phase high performance liquid chromatography was used to determine the content of purine adenylates, the TAN and AEC of the muscles of the both hind limbs were calculated, and the related regression equations of their relationship with PMI were established. Results Within 168 h of death of rats, the trend of ATP change was different from ADP, and the content of AMP continuously increased. The TAN value gradually increased with the extension of PMI, and the AEC showed a downward trend within 168 h after death. Among them, the patterns of AEC changes with PMI were obvious, the correlation coefficient was high ï¼ R2=0.903ï¼, and the curve fitting relationship was good; the fitting relationship between ATP, ADP, AMP, TAN and PMI was poor ï¼ R2=0.198-0.754ï¼. Conclusion The postmortem change patterns of AEC provide new research ideas for PMI estimation in the forensic field.
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Base de dados:
MEDLINE
Assunto principal:
Nucleotídeos de Adenina
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Músculos
Idioma:
En
/
Zh
Ano de publicação:
2021
Tipo de documento:
Article