Anti-CD20 therapy corrects a CD8 regulatory T cell deficit in multiple sclerosis.
Mult Scler
; 27(14): 2170-2179, 2021 12.
Article
em En
| MEDLINE
| ID: mdl-33783270
ABSTRACT
OBJECTIVE:
To determine the effect of long-term anti-CD20 B-cell-depleting treatment on regulatory T cell immune subsets that are subnormal in untreated MS patients.METHODS:
30 clinically stable MS patients, before and over 38 months of ocrelizumab treatment, were compared to 13 healthy controls, 29 therapy-naïve MS, 9 interferon-ß-treated MS, 3 rituximab-treated MS, and 3 rituximab-treated patients with other autoimmune inflammatory diseases. CD8, CD28, CD4, and FOXP3 expression in peripheral blood mononuclear cells was quantitated with flow cytometry.RESULTS:
CD8+ CD28- regulatory cells rose from one-third of healthy control levels before ocrelizumab treatment (2.68% vs 7.98%), normalized by 12 months (13.5%), and rose to 2.4-fold above healthy controls after 18 months of ocrelizumab therapy (19.0%). CD4+ FOXP3+ regulatory cells were lower in MS than in healthy controls (7.98%) and showed slight long-term decreases with ocrelizumab. CD8+ CD28- and CD4+ FOXP3+ regulatory T cell percentages in IFN-ß-treated MS patients were between those of untreated MS and healthy controls.INTERPRETATION:
Long-term treatment with ocrelizumab markedly enriches CD8+ CD28- regulatory T cells and corrects the low levels seen in MS before treatment, while slightly decreasing CD4+ FOXP3+ regulatory T cells. Homeostatic enrichment of regulatory CD8 T cells provides a mechanism, in addition to B cell depletion, for the benefits of anti-CD20 treatment in MS.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T Reguladores
/
Esclerose Múltipla
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article