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Five Commercial Immunoassays for SARS-CoV-2 Antibody Determination and Their Comparison and Correlation with the Virus Neutralization Test.
Simánek, Václav; Pecen, Ladislav; Krátká, Zuzana; Fürst, Tomás; Rezácková, Hana; Topolcan, Ondrej; Fajfrlík, Karel; Sedlácek, Dalibor; Sín, Robin; Pazdiora, Petr; Zelená, Hana; Slouka, David; Kucera, Radek.
Afiliação
  • Simánek V; Department of Immunochemistry Diagnostics, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic.
  • Pecen L; Department of Immunochemistry Diagnostics, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic.
  • Krátká Z; Laboratory of Immunology, Gennet, 110 00 Prague, Czech Republic.
  • Fürst T; Faculty of Science, Palacky University in Olomouc, 779 00 Olomouc, Czech Republic.
  • Rezácková H; Department of Immunochemistry Diagnostics, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic.
  • Topolcan O; Department of Immunochemistry Diagnostics, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic.
  • Fajfrlík K; Department of Microbiology, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic.
  • Sedlácek D; Department of Infectious Diseases and Travel Medicine, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic.
  • Sín R; Department of Infectious Diseases and Travel Medicine, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic.
  • Pazdiora P; Institute of Epidemiology, Faculty of Medicine in Pilsen, Charles University, 305 99 Pilsen, Czech Republic.
  • Zelená H; Department of Virology, Public Health Institute, 702 00 Ostrava, Czech Republic.
  • Slouka D; Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava, 703 00 Ostrava, Czech Republic.
  • Kucera R; Department of Otorhinolaryngology, University Hospital in Pilsen, Faculty of Medicine in Pilsen, Charles University, 305 99 Pilsen, Czech Republic.
Diagnostics (Basel) ; 11(4)2021 Mar 25.
Article em En | MEDLINE | ID: mdl-33806216
There is an ongoing debate as to whether SARS-CoV-2 antibodies can be found in patients who have recovered from COVID-19 disease. Currently, there is no consensus on whether the antibodies, if present, are protective. Our regular measurements of SARS-CoV-2 antibodies, starting in July 2020, have provided us with the opportunity of becoming acquainted with the five different immunoassays. A total of 149 patients were enrolled in our study. We measured the samples using each immunoassay, then performing a virus neutralization test and comparing the results of SARS-CoV-2 antibodies with this test. We observed that the production of neutralizing antibodies is age-dependent. Elderly patients have a higher proportion of high neutralizing titers than young patients. Based on our results, and in combination with the literature findings, we can conclude that the serological SARS-CoV-2 antibody measurement is a helpful tool in the fight against COVID-19. The assays can provide information about the patient's previous contact with the virus. Anti-spike protein assays correlate well with the virus neutralization test and can be used in the screening of potential convalescent plasma donors.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article