An Optimized Whole-Mount Immunofluorescence Method for Shoot Apices.
Curr Protoc
; 1(4): e101, 2021 Apr.
Article
em En
| MEDLINE
| ID: mdl-33826805
ABSTRACT
The localization of a protein provides important information about its biological functions. The visualization of proteins by immunofluorescence has become an essential approach in cell biology. Here, we describe an easy-to-follow immunofluorescence protocol to localize proteins in whole-mount tissues of maize (Zea mays) and Arabidopsis. We present the whole-mount immunofluorescence procedure using maize ear primordia and Arabidopsis inflorescence apices as examples, followed by tips and suggestions for each step. In addition, we provide a supporting protocol to describe the use of an ImageJ plug-in to analyze colocalization. This protocol has been optimized to observe proteins in 2-5 mm maize ear primordia or in developing Arabidopsis inflorescence apices; however, it can be used as a reference to perform whole-mount immunofluorescence in other plant tissues and species. © 2021 Wiley Periodicals LLC. Basic Protocol Whole-mount immunofluorescence for maize and Arabidopsis shoot apices Support Protocol Measure colocalization by JACoP plugin in ImageJ.
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MEDLINE
Assunto principal:
Arabidopsis
/
Proteínas de Arabidopsis
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article