Stem-loop-primer assisted isothermal amplification enabling high-specific and ultrasensitive nucleic acid detection.

Luo, Guangcheng; Yi, Tingting; Wang, Qiang; Guo, Bin; Fang, Li; Zhang, Guoyuan; Guo, Xiaolan

Luo, Guangcheng; Yi, Tingting; Wang, Qiang; Guo, Bin; Fang, Li; Zhang, Guoyuan; Guo, Xiaolan.

Afiliação

Luo G; Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, PR China; Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, 637000, PR China; Translational Medicine Research Center, North Sichuan Medical College, Nanchong, 637000,
Yi T; Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, PR China.
Wang Q; Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, 637000, PR China.
Guo B; Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, 637000, PR China.
Fang L; Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, 637000, PR China.
Zhang G; Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, PR China. Electronic address: 13508099826@126.com.
Guo X; Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, PR China; Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, 637000, PR China; Translational Medicine Research Center, North Sichuan Medical College, Nanchong, 637000,

Biosens Bioelectron ; 184: 113239, 2021 Jul 15.

Article em En | MEDLINE | ID: mdl-33857727

Assuntos

Técnicas Biossensoriais; Ácidos Nucleicos; Humanos; Técnicas de Diagnóstico Molecular; Técnicas de Amplificação de Ácido Nucleico; Sensibilidade e Especificidade

Palavras-chave

Colorimetric nucleic acid testing; Isothermal amplification technology; Point-of-care testing; Stem-loop primer; Ultralow background amplification

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos / Técnicas Biossensoriais Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos / Técnicas Biossensoriais Idioma: En Ano de publicação: 2021 Tipo de documento: Article