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Host barriers to SARS-CoV-2 demonstrated by ferrets in a high-exposure domestic setting.
Sawatzki, Kaitlin; Hill, Nichola J; Puryear, Wendy B; Foss, Alexa D; Stone, Jonathon J; Runstadler, Jonathan A.
Afiliação
  • Sawatzki K; Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536 kaitlin.sawatzki@tufts.edu.
  • Hill NJ; Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536.
  • Puryear WB; Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536.
  • Foss AD; Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536.
  • Stone JJ; Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536.
  • Runstadler JA; Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536.
Proc Natl Acad Sci U S A ; 118(18)2021 05 04.
Article em En | MEDLINE | ID: mdl-33858941
Ferrets (Mustela putorius furo) are mustelids of special relevance to laboratory studies of respiratory viruses and have been shown to be susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and onward transmission. Here, we report the results of a natural experiment where 29 ferrets in one home had prolonged, direct contact and constant environmental exposure to two humans with symptomatic disease, one of whom was confirmed positive for SARS-CoV-2. We observed no evidence of SARS-CoV-2 transmission from humans to ferrets based on viral and antibody assays. To better understand this discrepancy in experimental and natural infection in ferrets, we compared SARS-CoV-2 sequences from natural and experimental mustelid infections and identified two surface glycoprotein Spike (S) mutations associated with mustelids. While we found evidence that angiotensin-converting enzyme II provides a weak host barrier, one mutation only seen in ferrets is located in the novel S1/S2 cleavage site and is computationally predicted to decrease furin cleavage efficiency. These data support the idea that host factors interacting with the novel S1/S2 cleavage site may be a barrier in ferret SARS-CoV-2 susceptibility and that domestic ferrets are at low risk of natural infection from currently circulating SARS-CoV-2. We propose two mechanistically grounded hypotheses for mustelid host adaptation of SARS-CoV-2, with possible effects that require additional investigation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Furões / Glicoproteína da Espícula de Coronavírus / Adaptação ao Hospedeiro / SARS-CoV-2 / COVID-19 / Mutação Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Furões / Glicoproteína da Espícula de Coronavírus / Adaptação ao Hospedeiro / SARS-CoV-2 / COVID-19 / Mutação Idioma: En Ano de publicação: 2021 Tipo de documento: Article