LC-PDA-MS/MS-Based Characterization of Key Phytochemicals in Eurycoma Longifolia Roots.
J Chromatogr Sci
; 59(7): 659-669, 2021 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-33876232
ABSTRACT
The objective of the study was to fractionate the crude extract of Eurycoma longifolia (E. longifolia) roots and identify the intense peaks using HPLC-PDA-MS/MS, UPLC-MS/MS and H-NMR. Column chromatography was used to fractionate the crude extract into individual fractions using six solvent systems ranged from ethyl acetate, methanol and water in increasing polarity. Two fractions with nearly pure and intense peaks were selected for compound identification. Chromenone (coumarin) and chromone derivatives were putatively identified, besides several previously reported quassinoid glycosides (eurycomanone derived glycoside, 2,3-dehydro-4α-hydroxylongilactone glucoside, eurycomanol glycoside and eurycomanol trimer) in the fraction 11 of 100% methanol. A newly reported compound, namely hydroxyl glyyunanprosapogenin D (838 g/mol) was proposed to be the compound detected in the fraction 11 of 50% ethyl acetate and 50% methanol. This is also the first study to report the identification of chromenones and chromones in E. longifolia extract.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
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Raízes de Plantas
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Eurycoma
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Espectrometria de Massas em Tandem
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Compostos Fitoquímicos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article