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Conditional destabilization of the TPLATE complex impairs endocytic internalization.
Wang, Jie; Yperman, Klaas; Grones, Peter; Jiang, Qihang; Dragwidge, Jonathan; Mylle, Evelien; Mor, Eliana; Nolf, Jonah; Eeckhout, Dominique; De Jaeger, Geert; De Rybel, Bert; Pleskot, Roman; Van Damme, Daniel.
Afiliação
  • Wang J; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
  • Yperman K; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
  • Grones P; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
  • Jiang Q; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
  • Dragwidge J; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
  • Mylle E; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
  • Mor E; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
  • Nolf J; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
  • Eeckhout D; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
  • De Jaeger G; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
  • De Rybel B; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
  • Pleskot R; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
  • Van Damme D; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
Proc Natl Acad Sci U S A ; 118(15)2021 04 13.
Article em En | MEDLINE | ID: mdl-33876766
In plants, endocytosis is essential for many developmental and physiological processes, including regulation of growth and development, hormone perception, nutrient uptake, and defense against pathogens. Our toolbox to modulate this process is, however, rather limited. Here, we report a conditional tool to impair endocytosis. We generated a partially functional TPLATE allele by substituting the most conserved domain of the TPLATE subunit of the endocytic TPLATE complex (TPC). This substitution destabilizes TPC and dampens the efficiency of endocytosis. Short-term heat treatment increases TPC destabilization and reversibly delocalizes TPLATE from the plasma membrane to aggregates in the cytoplasm. This blocks FM uptake and causes accumulation of various known endocytic cargoes at the plasma membrane. Short-term heat treatment therefore transforms the partially functional TPLATE allele into an effective conditional tool to impair endocytosis. Next to their role in endocytosis, several TPC subunits are also implicated in actin-regulated autophagosomal degradation. Inactivating TPC via the WDX mutation, however, does not impair autophagy, thus enabling specific and reversible modulation of endocytosis in planta.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Arabidopsis / Endocitose Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Arabidopsis / Endocitose Idioma: En Ano de publicação: 2021 Tipo de documento: Article