Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies.
J Vet Intern Med
; 35(3): 1288-1296, 2021 May.
Article
em En
| MEDLINE
| ID: mdl-33955083
ABSTRACT
BACKGROUND:
In inflammatory bowel disease (IBD) in humans, phosphorylated signal transducer and activator of transcription 3 (pSTAT3) is upregulated in mucosal epithelial cells and correlates with clinical severity. HYPOTHESIS/OBJECTIVE:
To investigate the expression pattern of pSTAT3 in the mucosa of dogs with chronic inflammatory enteropathy (CIE) and explore correlations between its expression and clinical and histopathological severity scoring. ANIMALS Twenty-eight canine CIE patients grouped into food-responsive enteropathy (FRE; 9), steroid-responsive enteropathy (SRE; 10), and protein-losing enteropathy (PLE; 9). Ten healthy beagle dogs served as controls (CO).METHODS:
Retrospective case control study. Immunohistochemistry was used to detect pSTAT3 in canine duodenal mucosa samples.RESULTS:
Compared to CO, SRE (P < .001) and PLE (P < .001) dogs had significantly higher pSTAT3 expression in the villus epithelium. The SRE group had a significantly higher expression in the villus lamina propria (VLP) compared to controls (P = .009). In the crypt epithelium (CE), all CIE dogs had significantly higher pSTAT3 expression (FRE, P = .002; SRE, P = .003; PLE, P < .001) compared to CO. In the lamina propria crypt region (CLP), dogs with FRE (P = .04) and SRE (P = .03) had significantly upregulated pSTAT3 compared to controls. A positive correlation was found between canine chronic enteropathy clinical activity index (CCECAI) scoring and pSTAT3 expression for both epithelial (rho = .541; P < .001) and crypt regions (rho = .32; P = .02). CONCLUSIONS AND CLINICAL IMPORTANCE pSTAT3 is upregulated in CIE in dogs, correlates with clinical severity, and may be helpful as a clinical marker in dogs with CIE.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Enteropatias Perdedoras de Proteínas
/
Doenças Inflamatórias Intestinais
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Doenças do Cão
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article