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Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage.
Sojitra, Mirat; Sarkar, Susmita; Maghera, Jasmine; Rodrigues, Emily; Carpenter, Eric J; Seth, Shaurya; Ferrer Vinals, Daniel; Bennett, Nicholas J; Reddy, Revathi; Khalil, Amira; Xue, Xiaochao; Bell, Michael R; Zheng, Ruixiang Blake; Zhang, Ping; Nycholat, Corwin; Bailey, Justin J; Ling, Chang-Chun; Lowary, Todd L; Paulson, James C; Macauley, Matthew S; Derda, Ratmir.
Afiliação
  • Sojitra M; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Sarkar S; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Maghera J; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Rodrigues E; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Carpenter EJ; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Seth S; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Ferrer Vinals D; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Bennett NJ; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Reddy R; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Khalil A; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Xue X; Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, El Sherouk, Egypt.
  • Bell MR; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Zheng RB; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Zhang P; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Nycholat C; Department of Chemistry, University of Calgary, Calgary, Alberta, Canada.
  • Bailey JJ; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA.
  • Ling CC; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Lowary TL; Department of Chemistry, University of Calgary, Calgary, Alberta, Canada.
  • Paulson JC; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Macauley MS; Institute of Biological Chemistry, Academia Sinica, Nangang, Taipei, Taiwan.
  • Derda R; Institute of Biochemical Sciences, National Taiwan University, Da'an, Taipei, Taiwan.
Nat Chem Biol ; 17(7): 806-816, 2021 07.
Article em En | MEDLINE | ID: mdl-33958792
ABSTRACT
The central dogma of biology does not allow for the study of glycans using DNA sequencing. We report a liquid glycan array (LiGA) platform comprising a library of DNA 'barcoded' M13 virions that display 30-1,500 copies of glycans per phage. A LiGA is synthesized by acylation of the phage pVIII protein with a dibenzocyclooctyne, followed by ligation of azido-modified glycans. Pulldown of the LiGA with lectins followed by deep sequencing of the barcodes in the bound phage decodes the optimal structure and density of the recognized glycans. The LiGA is target agnostic and can measure the glycan-binding profile of lectins, such as CD22, on cells in vitro and immune cells in a live mouse. From a mixture of multivalent glycan probes, LiGAs identify the glycoconjugates with optimal avidity necessary for binding to lectins on living cells in vitro and in vivo.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Bacteriófago M13 / Análise em Microsséries Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Bacteriófago M13 / Análise em Microsséries Idioma: En Ano de publicação: 2021 Tipo de documento: Article