Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing.
ACS Nano
; 15(5): 8517-8524, 2021 05 25.
Article
em En
| MEDLINE
| ID: mdl-33961404
ABSTRACT
The dynamic nature of micellar nanostructures is employed to form a self-assembled Förster resonance energy transfer (FRET) nanoplatform for enhanced sensing of DNA. The platform consists of lipid oligonucleotide FRET probes incorporated into micellar scaffolds, where single recognition events result in fusion and fission of DNA mixed micelles, triggering the fluorescence response of multiple rather than a single FRET pair. In comparison to conventional FRET substrates where a single donor interacts with a single acceptor, the micellar multiplex FRET system showed â¼20- and â¼3-fold enhancements in the limit of detection and FRET efficiency, respectively. This supramolecular signal amplification approach could potentially be used to improve FRET-based diagnostic assays of nucleic acid and non-DNA based targets.
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Nucleicos
/
Nanoestruturas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article