Programmable Aggregation of Artificial Cells with DNA Signals.
ACS Synth Biol
; 10(6): 1268-1276, 2021 06 18.
Article
em En
| MEDLINE
| ID: mdl-34006093
ABSTRACT
Cell aggregation is a complex behavior that is closely related to the viability, differentiation, and migration of cells. An effort to create synthetic analogs could lead to considerable advances in cell physiology and biophysics. Rendering and modulating such a dynamic artificial cell system require mechanisms for receiving, transducing, and transmitting intercellular signals, yet effective tools are limited at present. Here we construct synthetic cells from engineered lipids and show their programmable aggregation behaviors using DNA oligonucleotides as signaling molecules. The artificial cells have transmembrane channels made of DNA origami that are used to recognize and process intercellular signals. We demonstrate that multiple small vesicles aggregate onto a giant vesicle after a transduction of external DNA signals by an intracellular enzyme and that the small vesicles dissociate when receiving "release" signals. This work provides new possibilities for building synthetic protocells capable of chemical communication and coordination.
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Base de dados:
MEDLINE
Assunto principal:
DNA
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Transdução de Sinais
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Agregação Celular
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Células Artificiais
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article