Fine Sampling of Sequence Space for Membrane Protein Structural Biology.
J Mol Biol
; 433(15): 167055, 2021 07 23.
Article
em En
| MEDLINE
| ID: mdl-34022208
ABSTRACT
We describe an enhancement of traditional genomics-based approaches to improve the success of structure determination of membrane proteins. Following a broad screen of sequence space to identify initial expression-positive targets, we employ a second step to select orthologs with closely related sequences to these hits. We demonstrate that a greater percentage of these latter targets express well and are stable in detergent, increasing the likelihood of identifying candidates that will ultimately yield structural information.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article