α-Synuclein Seeding Assay Using Cultured Cells.
Methods Mol Biol
; 2322: 27-39, 2021.
Article
em En
| MEDLINE
| ID: mdl-34043189
ABSTRACT
α-Synuclein, a presynaptic protein, is involved in synaptic vesicle dynamics in response to neuronal activity. Mutations of the α-synuclein gene and the neuronal deposition of α-synuclein, called Lewy bodies, are linked to the development of Parkinson's disease. α-Synuclein has a prion-like property that converts its physiological protein conformation to a pathogenic one, forming disease-causing fibrils. Aggregation of these fibrils and subsequent inclusion formation are suggested to interfere with vesicular trafficking and organelle function in neurons. Thus, detection of a prion-like property of α-synuclein and the evaluation of its modifying factors are required to understand the pathogenesis of Parkinson's disease and to develop new therapies. In this chapter, we describe a cell-based assay for detecting α-synuclein propagation.
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Base de dados:
MEDLINE
Assunto principal:
Células Cultivadas
/
Alfa-Sinucleína
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article