Cytosolic and Mitochondrial Ca2+ Imaging in Drosophila Dopaminergic Neurons.
Methods Mol Biol
; 2322: 207-214, 2021.
Article
em En
| MEDLINE
| ID: mdl-34043206
ABSTRACT
The ATP-producing organelle mitochondrion controls cellular or synaptic Ca2+ concentrations through temporal uptake of Ca2+ outside of the mitochondria. Although intracellular Ca2+ influx occurs during neuronal activity, a persistently higher concentration of intracellular Ca2+ is neurotoxic. Healthy mitochondria ensure rapid Ca2+ uptake, which is necessary for proper neuronal activity. Mitochondrial Ca2+ buffering activity decreases in aged or sick neurons. In this chapter, we will introduce our protocol for evaluating Ca2+ buffering activity through the mitochondria during neuronal activity of dopaminergic neurons.
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Base de dados:
MEDLINE
Assunto principal:
Cálcio
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Sinalização do Cálcio
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Citosol
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Drosophila
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Neurônios Dopaminérgicos
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Mitocôndrias
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article