Sphingobacterium lumbrici sp. nov., a novel bacterium isolated from wormcast of Eisenia foetida.
Int J Syst Evol Microbiol
; 71(5)2021 May.
Article
em En
| MEDLINE
| ID: mdl-34047689
ABSTRACT
A novel Gram-stain-negative, rod-shaped, non-motile, yellowish bacterium, designated strain 1.3611T, was isolated from the wormcast of Eisenia foetida. The strain grew optimally at 30-37 â, at pH 7.0 and with 0-1.0â% (w/v) NaCl. Based on the results of 16S rRNA gene sequence and phylogenetic analyses, strain 1.3611T showed the highest degree of 16S rRNA gene sequence similarity to Sphingobacterium olei HAL-9T (97.0â%), followed by Sphingobacterium alkalisoli Y3L14T (95.8â%). The respiratory quinone of strain 1.3611T was menaquinone-7 (MK-7) and its major cellular fatty acids were iso-C15ââ0 (41.3â%), summed feature 3 (C16ââ1 ω7c and/or C16ââ1 ω6c, 22.1â%) and iso-C17ââ0 3-OH (16.2â%). The major polar lipids were sphingophospholipid, phosphatidylethanolamine, four unidentified glycolipids, two unidentified phospholipids and five unidentified polar lipids. The genomic DNA G+C content was 39.0âmol%. The digital DNA-DNA hybridization and average nucleotide identity values between the genomes of strain 1.3611T and S. olei HAL-9T were 37.9 and 88.9â%, respectively. According to the phenotypic and chemotaxonomic phylogenetic results, strain 1.3611T should represent a novel species of the genus Sphingobacterium, for which the name Sphingobacterium lumbrici sp. nov. is proposed, with strain 1.3611T (=KCTC 62980T=CCTCC AB 2018349T) as the type strain.
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MEDLINE
Assunto principal:
Oligoquetos
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Filogenia
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Sphingobacterium
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article