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Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins.
Robeck, Todd R; Fei, Zhe; Lu, Ake T; Haghani, Amin; Jourdain, Eve; Zoller, Joseph A; Li, Caesar Z; Steinman, Karen J; DiRocco, Stacy; Schmitt, Todd; Osborn, Steve; Van Bonn, Bill; Katsumata, Etsuko; Mergl, June; Almunia, Javier; Rodriguez, Magdalena; Haulena, Martin; Dold, Christopher; Horvath, Steve.
Afiliação
  • Robeck TR; Zoological Operations, SeaWorld Parks and Entertainment, Orlando, FL, USA. todd.robeck@seaworld.com.
  • Fei Z; Department of Biostatistics, Fielding School of Public Health, University of California, Los Angeles, Los Angeles, CA, USA.
  • Lu AT; Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
  • Haghani A; Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
  • Jourdain E; Norwegian Orca Survey, Andenes, Norway.
  • Zoller JA; Department of Biostatistics, Fielding School of Public Health, University of California, Los Angeles, Los Angeles, CA, USA.
  • Li CZ; Department of Biostatistics, Fielding School of Public Health, University of California, Los Angeles, Los Angeles, CA, USA.
  • Steinman KJ; Species Preservation Laboratory, SeaWorld San Diego, San Diego, CA, USA.
  • DiRocco S; SeaWorld Orlando, Orlando, FL, USA.
  • Schmitt T; SeaWorld San Diego, San Diego, CA, USA.
  • Osborn S; SeaWorld San Antonio, San Antonio, TX, USA.
  • Van Bonn B; John G. Shedd Aquarium, Chicago, IL, USA.
  • Katsumata E; Kamogawa Sea World, Kamogawa, Chiba, Japan.
  • Mergl J; Marineland of Canada, Niagara Falls, ON, Canada.
  • Almunia J; Loro Parque Fundación, SA, Avenida Loro Parque, Puerto de la Cruz, Santa Cruz de Tenerife, Spain.
  • Rodriguez M; Miami Seaquarium, Miami, FL, USA.
  • Haulena M; Vancouver Aquarium, Vancouver, Canada.
  • Dold C; Zoological Operations, SeaWorld Parks and Entertainment, Orlando, FL, USA.
  • Horvath S; Department of Biostatistics, Fielding School of Public Health, University of California, Los Angeles, Los Angeles, CA, USA. shorvath@mednet.ucla.edu.
Commun Biol ; 4(1): 642, 2021 05 31.
Article em En | MEDLINE | ID: mdl-34059764
ABSTRACT
The development of a precise blood or skin tissue DNA Epigenetic Aging Clock for Odontocete (OEAC) would solve current age estimation inaccuracies for wild odontocetes. Therefore, we determined genome-wide DNA methylation profiles using a custom array (HorvathMammalMethyl40) across skin and blood samples (n = 446) from known age animals representing nine odontocete species within 4 phylogenetic families to identify age associated CG dinucleotides (CpGs). The top CpGs were used to create a cross-validated OEAC clock which was highly correlated for individuals (r = 0.94) and for unique species (median r = 0.93). Finally, we applied the OEAC for estimating the age and sex of 22 wild Norwegian killer whales. DNA methylation patterns of age associated CpGs are highly conserved across odontocetes. These similarities allowed us to develop an odontocete epigenetic aging clock (OEAC) which can be used for species conservation efforts by provide a mechanism for estimating the age of free ranging odontocetes from either blood or skin samples.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Baleias / Envelhecimento / Golfinhos Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Baleias / Envelhecimento / Golfinhos Idioma: En Ano de publicação: 2021 Tipo de documento: Article