circNSUN2 promotes the malignant biological behavior of colorectal cancer cells via the miR181a5p/ROCK2 axis.
Oncol Rep
; 46(1)2021 07.
Article
em En
| MEDLINE
| ID: mdl-34080658
ABSTRACT
Aberrant expression of circular RNAs (circRNAs) has been demonstrated to be related to the development of colorectal cancer (CRC), the third most common cancer worldwide. However, the mechanism of the effect of circRNA NOP2/Sun domain family, member 2 (circNSUN2) on the malignant biological behavior of CRC remains unclear. In the present study, the expression of circNSUN2 and microRNA (miR)181a5p was detected by RTqPCR. The expression of Rhoassociated coiledcoilcontaining protein kinase 2 (ROCK2) was measured by western blotting. Cell proliferation was detected by CCK8 assay. The cell apoptosis rate was measured by flow cytometry. Cell migration ability was evaluated by Transwell assay. The interactions between circNSUN2, miR181a5p and ROCK2 were verified by dualluciferase reporter assay. The results revealed that circNSUN2 was highly expressed in CRC tissues and cell lines. Knockdown of circNSUN2 inhibited the malignant biological behavior of CRC in vivo and in vitro. Moreover, miR181a5p was revealed to be a target gene of circNSUN2, and the expression of ROCK2 was negatively regulated by miR181a5p. Knockdown of circNSUN2 inhibited proliferation and migration, and induced apoptosis of CRC cells and suppressed tumor growth by targeting miR181a5p to decrease ROCK2 expression. In conclusion, circNSUN2 promoted the progression of CRC by sponging miR181a5p to increase the expression of ROCK2.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
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MicroRNAs
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Quinases Associadas a rho
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RNA Circular
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article