An ALA122 THR substitution in the AHAS/ALS gene confers imazamox-resistance in Aegilops cylindrica.
Pest Manag Sci
; 77(10): 4583-4592, 2021 Oct.
Article
em En
| MEDLINE
| ID: mdl-34087037
ABSTRACT
BACKGROUND:
Wheat growers have limited herbicide options to manage Aegilops cylindrica Host (jointed goatgrass), with many relying on mesosulfuron or imazamox in Clearfield™ winter wheat. Both imazamox and mesosulfuron inhibit acetohydroxyacid synthase/acetolactate synthase (AHAS/ALS). In 2015, a suspected imazamox resistant biotype of Ae. cylindrica was found in eastern Washington.RESULTS:
Imazamox and mesosulfuron were applied to the suspected resistant and susceptible Ae. cylindrica biotypes in increasing application rates to evaluate herbicide dose needed to cause 50% growth reduction (GR50 ). The imazamox resistant biotype had a GR50 of 308.5 g ai ha-1 and was more than 5000 times more resistant to imazamox than a known susceptible biotype with a GR50 of 0.06 g ai ha-1 . The Ae. cylindrica resistant biotype was also resistant to mesosulfuron, with an GR50 of 46.82 g ai ha-1 , which was five times more than the susceptible GR50 of 8.6 g ai ha-1 . Sequencing of the AHAS/ALS gene revealed an Ala122 Thr substitution in the herbicide binding region of the AHAS/ALS gene on the D genome of Ae. cylindrica. The resistance trait was inherited as a dominant trait, and the Ala122 Thr co-segregates with the resistance phenotype.CONCLUSIONS:
An Ala122 Thr substitution in the AHAS/ALS gene on the D genome of Ae. cylindrica confers resistance to imazamox in Ae. cylindrica. © 2021 Society of Chemical Industry.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acetolactato Sintase
/
Aegilops
/
Herbicidas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article