Quantifying and visualising the nuances of cellular dynamics in vivo using intravital imaging.
Curr Opin Cell Biol
; 72: 41-53, 2021 10.
Article
em En
| MEDLINE
| ID: mdl-34091131
ABSTRACT
Intravital imaging is a powerful technology used to quantify and track dynamic changes in live cells and tissues within an intact environment. The ability to watch cell biology in real-time 'as it happens' has provided novel insight into tissue homeostasis, as well as disease initiation, progression and response to treatment. In this minireview, we highlight recent advances in the field of intravital microscopy, touching upon advances in awake versus anaesthesia-based approaches, as well as the integration of biosensors into intravital imaging. We also discuss current challenges that, in our opinion, need to be overcome to further advance the field of intravital imaging at the single-cell, subcellular and molecular resolution to reveal nuances of cell behaviour that can be targeted in complex disease settings.
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1
Base de dados:
MEDLINE
Assunto principal:
Microscopia Intravital
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article