E2F2 inhibition induces autophagy via the PI3K/Akt/mTOR pathway in gastric cancer.
Aging (Albany NY)
; 13(10): 13626-13643, 2021 04 21.
Article
em En
| MEDLINE
| ID: mdl-34091441
ABSTRACT
BACKGROUND:
E2F2 is a member of the E2F transcription factor family and has important but not fully understood biological functions in cancers. The biological role of E2F2 in gastric cancer (GC) also remains unclear.METHODS:
We examined the expression levels of E2F2 in GC using publicly available datasets such as TIMER, Oncomine, GEPIA, UALCAN, etc., and in our patient cohort, using quantitative real-time PCR, western blotting, and immunohistochemistry. We further investigated the effects of E2F2 on phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling, autophagy, and the migration and invasion of GC cells by the wound healing assay, Transwell assay and transmission electron microscopy.RESULTS:
E2F2 was highly expressed in both GC tissues and cells compared with normal gastric tissues/cells. High E2F2 expression was associated with poor overall survival (OS). In addition, the expression of E2F2 in GC was strongly correlated with a variety of immune markers. E2F2 overexpression promoted the migration and invasiveness of GC cells in vitro through inhibition of PI3K/Akt/mTOR-mediated autophagy.CONCLUSION:
High E2F2 expression was associated with the characteristics of invasive tumors and poor prognosis. E2F2 also had potential modulatory effects on tumor immunity. We discovered a novel function of E2F2 in the regulation of PI3K/Akt/mTOR-mediated autophagy and the downstream processes of cell migration and invasion.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Autofagia
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Neoplasias Gástricas
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Transdução de Sinais
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Proteínas Proto-Oncogênicas c-akt
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Fator de Transcrição E2F2
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Fosfatidilinositol 3-Quinase
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Serina-Treonina Quinases TOR
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article