miRNAs through ß-ARR2/p-ERK1/2 pathway regulate the VSMC proliferation and migration.
Life Sci
; 279: 119703, 2021 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-34111458
ABSTRACT
BACKGROUND:
miRNAs are involved in plaque formation of atherosclerosis and vessel restenosis. In this study, we investigated the effects of miR-599, miR-204, and miR-181b on VSMC proliferation, and migration through TGFß receptor 2 (TGFßR2), ß-arrestin 2 (ß-ARR2), SMAD2/p-SMAD2, and ERK1/2/p-ERK1/2. MATERIALS &METHODS:
Genes and miRNAs were predicted by bioinformatics tools and were transfected by PEI-miRNAs (miR-599, miR-204, and miR-181b) complexes into VSMCs. The gene and protein expression levels were evaluated by real-time RT-PCR and western blotting techniques, respectively. The VSMC proliferation and migration were studied by MTT and scratch assay, respectively.RESULTS:
The miR-181b and miR-204 downregulated significantly ß-ARR2 gene and protein expression levels and p-ERK1/2 values. Moreover, TGFßR2 gene and protein expression levels and p-SMAD2 values were not significantly affected by miR-181b and miR-204. The VSMC proliferation (p = 0.0019, p = 0.0054, respectively) and migration (p < 0.0001, p < 0.0001, respectively) were inhibited by the miR-181b and miR-204. The miR-599 inhibited VSMC proliferation (p = 0.044) and migration (p = 0.0055) but it did not affect significantly the ß-ARR2 and TGFßR2 gene and protein expression levels.CONCLUSION:
The results suggested that the inhibitory effects of miR-181b and miR-204 on VSMC proliferation and migration are mediated by the ß-ARR2/p-ERK1/2 pathway. Since VSMC proliferation and migration are involved in plaque growth, therefore this pathway can be a therapeutic target for atherosclerosis.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Movimento Celular
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Regulação da Expressão Gênica
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Miócitos de Músculo Liso
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MicroRNAs
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Proliferação de Células
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Músculo Liso Vascular
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article