Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography -Tandem Mass Spectrometry.
Methods Mol Biol
; 2275: 329-339, 2021.
Article
em En
| MEDLINE
| ID: mdl-34118048
ABSTRACT
Coenzyme Q10 (CoQ10) is an essential part of the mitochondrial respiratory chain . Here, we describe an accurate and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of mitochondrial CoQ10 in isolated mitochondria . In the assay, mitochondrial suspensions are spiked with CoQ10-[2H9] internal standard (IS), extracted with organic solvents and CoQ10 quantified by LC-MS/MS using multiple reaction monitoring (MRM).
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Base de dados:
MEDLINE
Assunto principal:
Ubiquinona
/
Mitocôndrias Musculares
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article